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bacteria mini kit  (Qiagen)


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    Structured Review

    Qiagen bacteria mini kit
    Bacteria Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 815 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacteria+mini+kit/RNeasy+Protect+Bacteria+Mini+Kit/pm42143132-234-10-13
    Average 96 stars, based on 815 article reviews
    bacteria mini kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Production of lactase enzymes using altered regulation strains
    Article Snippet: A 1:2 volume of cell culture to RNAprotect (Qiagen) was rigorously mixed at cell harvest, centrifuged at 10,000 g, liquid was aspirated, and cell pellets stored at −80° C. Cells pellet was dissolved in TE buffer containing lysozyme (15 mg/mL), proteinase K (20 mg/mL) and mutanolysin (250 U/λL) and incubated at 37° C. shaking at 1400 rpm for 10 minutes. .. The subsequent RNA extraction procedure was performed with the RNeasy Protect Bacteria Mini Kit (Qiagen) as per the manufacturer's instructions, including removal of DNA with DNAse I. .. Total RNA was evaluated with a Bioanalyzer (Agilent) and ribosomal RNA depletion and 50 bp sequencing (Illumina) thereafter performed at Genewiz (USA).

    Article Title: Characterization of RcgA and RcgR, two rhizobial proteins involved in the modulation of plasmid transfer
    Article Snippet: Then, RNAprotect reagent (Qiagen, Hilden, Germany) was added, cells were harvested, and the pellets were frozen in liquid nitrogen. .. The commercial RNeasy R Protect Bacteria Mini Kit (Qiagen, Hilden, Germany) was used for the RNA extraction, and DNA was removed with DNase I (Qiagen, Hilden, Germany). .. Ribosomal RNA depletion was performed with a commercial kit (Ribo-Zero rRNA Removal Kit, Illumina Inc., San Diego, USA).

    Article Title: Characterization of RcgA and RcgR, two rhizobial proteins involved in the modulation of plasmid transfer.
    Article Snippet: Then, RNAprotect reagent (Qiagen, Hilden, Germany) was added, cells were harvested, and the pellets were frozen in liquid nitrogen. .. The commercial RNeasy R Protect Bacteria Mini Kit (Qiagen, Hilden, Germany) was used for the RNA extraction, and DNA was removed with DNase I (Qiagen, Hilden, Germany). .. Ribosomal RNA depletion was performed with a commercial kit (Ribo-Zero rRNA Removal Kit, Illumina Inc., San Diego, USA).

    Bacteria:

    Article Title: Production of lactase enzymes using altered regulation strains
    Article Snippet: A 1:2 volume of cell culture to RNAprotect (Qiagen) was rigorously mixed at cell harvest, centrifuged at 10,000 g, liquid was aspirated, and cell pellets stored at −80° C. Cells pellet was dissolved in TE buffer containing lysozyme (15 mg/mL), proteinase K (20 mg/mL) and mutanolysin (250 U/λL) and incubated at 37° C. shaking at 1400 rpm for 10 minutes. .. The subsequent RNA extraction procedure was performed with the RNeasy Protect Bacteria Mini Kit (Qiagen) as per the manufacturer's instructions, including removal of DNA with DNAse I. .. Total RNA was evaluated with a Bioanalyzer (Agilent) and ribosomal RNA depletion and 50 bp sequencing (Illumina) thereafter performed at Genewiz (USA).

    Article Title: Dissecting host stress responses for predictable heterologous gene expression in E. coli
    Article Snippet: Cell culture was mixed with RNAprotect Bacteria Reagent (Qiagen) in a 1:2 volume ratio, vortexed, and incubated at room temperature for 5 min. Pelleted cells were resuspended in 400 μl elution buffer, split into two aliquots, and stored at −80°C. .. Total RNA was extracted and purified using the RNeasy Protect Bacteria Mini Kit (Qiagen) on a QIAcube Connect (Qiagen). .. Lysozyme was used for the enzymatic lysis of cells, together with Proteinase K for degrading protein, and SUPERase•In RNAse inhibitor (Thermo Fisher) for protecting RNA.

    Article Title: Exopolysaccharide biosynthesis and regulation in Leuconostoc mesenteroides influences water holding capacity of fermented plant-based milks.
    Article Snippet: Cells were collected by centrifugation at 5000 ×g for 10 min and resuspended in RNase-free TE buffer (Sigma-Aldrich, USA) containing 15 mg/mL lysozyme (Merck, USA) and 10% Proteinase K (QIAGEN). .. Total RNA was then extracted using the RNeasy Protect Bacteria Mini Kit (QIAGEN, Germany). .. Genomic DNA contamination was eliminated with the RNase-free DNase Set (QIAGEN).

    Article Title: Gene expression plasticity under multiple stresses drives higher tolerance to a macrolide in saline and warmer environments.
    Article Snippet: .. RNA was extracted and purified using the RNeasy (R) Protect Bacteria Mini Kit (QIAGEN, Germany) with a previous digestion with lysozyme and proteinase K. Briefly, pellets were lysed during 10 min with agitation in TE buffer containing 20 mg mL–1 proteinase K and 15 mg mL–1 lysozyme. ..

    Article Title: Characterization of adaptive evolution strains for the development of triclosan resistance in Agrobacterium tumefaciens C58.
    Article Snippet: .. Total RNA samples were extracted from three independent biological replicates of each strain using the RNeasy Protect Bacteria Mini Kit (Qiagen) following the manufacturer’s protocol. .. The RNA was purified with VAHTS RNA clean beads (Vazyme #N412, Cellagen Technology, USA), and its quality and quantity were determined using an Agilent 2100/2200 Bioanalyzer (Agilent Technologies, Palo Alto, California, USA).

    Article Title: Atopic Dermatitis–like mouse model using early inoculation of patient-derived S. aureus together with MC903
    Article Snippet: .. Total RNA was extracted from mouse skin tissue and bacterial pellets (grown in TSB for 18 hours) using the miRNeasy Micro Kit (number 217084, Qiagen) and RNeasy Protect Bacteria Mini Kit (number 74524, Qiagen), respectively. .. RNA concentration and purity were assessed with a Nanodrop. cDNA was synthesized from 500 ng of RNA using the RevertAid First Strand cDNA Synthesis Kit (K1621, Thermo Fisher Scientific) with random hexamer primers, following the manufacturer’s instructions. qPCR was performed using Bio-Rad CFX96 and GoTag qPCR Master Mix (A6001, Promega).

    Article Title: Characterization of RcgA and RcgR, two rhizobial proteins involved in the modulation of plasmid transfer
    Article Snippet: Then, RNAprotect reagent (Qiagen, Hilden, Germany) was added, cells were harvested, and the pellets were frozen in liquid nitrogen. .. The commercial RNeasy R Protect Bacteria Mini Kit (Qiagen, Hilden, Germany) was used for the RNA extraction, and DNA was removed with DNase I (Qiagen, Hilden, Germany). .. Ribosomal RNA depletion was performed with a commercial kit (Ribo-Zero rRNA Removal Kit, Illumina Inc., San Diego, USA).

    Article Title: Characterization of RcgA and RcgR, two rhizobial proteins involved in the modulation of plasmid transfer.
    Article Snippet: Then, RNAprotect reagent (Qiagen, Hilden, Germany) was added, cells were harvested, and the pellets were frozen in liquid nitrogen. .. The commercial RNeasy R Protect Bacteria Mini Kit (Qiagen, Hilden, Germany) was used for the RNA extraction, and DNA was removed with DNase I (Qiagen, Hilden, Germany). .. Ribosomal RNA depletion was performed with a commercial kit (Ribo-Zero rRNA Removal Kit, Illumina Inc., San Diego, USA).

    Purification:

    Article Title: Dissecting host stress responses for predictable heterologous gene expression in E. coli
    Article Snippet: Cell culture was mixed with RNAprotect Bacteria Reagent (Qiagen) in a 1:2 volume ratio, vortexed, and incubated at room temperature for 5 min. Pelleted cells were resuspended in 400 μl elution buffer, split into two aliquots, and stored at −80°C. .. Total RNA was extracted and purified using the RNeasy Protect Bacteria Mini Kit (Qiagen) on a QIAcube Connect (Qiagen). .. Lysozyme was used for the enzymatic lysis of cells, together with Proteinase K for degrading protein, and SUPERase•In RNAse inhibitor (Thermo Fisher) for protecting RNA.

    Article Title: Gene expression plasticity under multiple stresses drives higher tolerance to a macrolide in saline and warmer environments.
    Article Snippet: .. RNA was extracted and purified using the RNeasy (R) Protect Bacteria Mini Kit (QIAGEN, Germany) with a previous digestion with lysozyme and proteinase K. Briefly, pellets were lysed during 10 min with agitation in TE buffer containing 20 mg mL–1 proteinase K and 15 mg mL–1 lysozyme. ..



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